【摘要】 目的建立血液中丙泊酚的气相色谱-质谱联用(GC-MS)分析方法。方法血液以2,4-二甲基-6-叔丁基苯酚为内标,用乙醚进行液液萃取,离心后取有机层,水浴下挥干,GC-MS检测。结果血液中丙泊酚与内标分离良好,在0.02~10μg/mL范围内线性良好,线性方程为y=0.3136x-0.0068,相关系数为0.9997,日内精密度与日间精密度(RSD)均小于4.8%,检出限(LOD)为0.005μg/mL。利用该方法测得实际案例血液中丙泊酚质量浓度为0.14μg/mL。结论建立的GC-MS方法简单、灵敏、可靠,可适用于血液中丙泊酚的法医毒物分析和临床药物监测。更多还原

【Abstract】 Objective To establish a gas chromatography-mass spectrometry(GC-MS) method for determination of propofol in human blood.Methods Propofol and 2-(tert-Butyl)-4,6-dimethylphenol(internal standard) were isolated from human blood samples with liquid-liquid ether extraction.The organic layer was collected after centrifugation and dried using the water bath.The extracted residue was analyzed by GC-MS.Results Propofol and the internal standard showed a good separation with a good linear concentration ranged from 0.02 to 10 g/mL in blood.The linear function was y=0.313 6 x-0.006 8 with the correlation coefficient of 0.999 7.The precision of intra-day and inter-day were less than 4.8% and the lower limit of detection of propofol was 0.005 g/mL.Propofol concentration of blood was 0.14 g/mL using this method in the practice work.Conclusion The GC-MS method is rapid,sensitive,reliable and suitable for qualitative and quantitative analysis propofol of blood in forensic toxicological analysis and clinical drug monitoring.更多还原