【摘要】 为了研究热激同源蛋白70(70 kDa heat shock cognate,HSC70)在孔雀鱼(Poecilia reticulata Peters)对环境适应中的作用,采用电子克隆与传统实验克隆相结合的技术,分析了孔雀鱼的一个HSC70基因(命名为PrHSC70)。PrHSC70基因包含一个长1941 bp的完整编码区,含有8个内含子。PrHSC70蛋白具有HSP70蛋白家族的特征序列,且与其他动物HSC70和HSP70都高度相似。在进化树上PrHSC70与所有鱼类HSC70聚为一簇,与它们的氨基酸序列一致性为93.2%～98.9%。更多还原

【Abstract】 To investigate the contribution of HSC70(70 kDa heat shock cognate) to the adaptability in Poecilia reticulata Peters,one HSC70 gene(designated as PrHSC70) was isolated from P.reticulata by in silico cloning and traditional RT-PCR amplification technique.The PrHSC70 gene contained 8 introns and a complete coding sequence of 1941 bp.The deduced amino acid sequence of PrHSC70 contained three signature motifs of the HSP70 family,and shared high identity with those of HSP70s and HSC70s from other organisms.The PrHSC70 showed the highest amino acid sequence identity(93.2%~98.9%) with HSC70s from other fishes,and clustered together with them in the phylogenetic tree.更多还原