【摘要】 利用cDNA-AFLP技术对小黑杨茎、叶基因的差异表达进行分析。结果表明,64对引物组合在茎和叶中共扩增出4 192条带,其中差异条带2 275条。通过对茎中特异表达基因的克隆和测序,获得了4条cDNA序列。经Blast-x比对分析表明,它们分别是类伸展蛋白、漆酶、过氧化物酶和细胞壁相关水解酶。进行RT-PCR分析发现,4个基因在茎中的表达水平明显高于叶和根。其中,以漆酶的基因片段为基础进行生物信息学分析和设计引物,用RT-PCR技术克隆到了1 413 bp包含开放阅读编码框的cDNA片段,其开放阅读编码框长度为1 020bp,编码339个氨基酸残基,与来自其他植物漆酶的氨基酸序列具有较高的同源性。更多还原

【Abstract】 Different expressed genes between stems and leaves of Populus simonii × P.nigra were observed by the cDNA-AFLP method.Result showed that a total of 4 192 fragments were amplified from the cDNA of stems and leaves,and a total of 2 275 fragments were differential displayed genes between stems and leaves.Four fragments from stems were cloned and sequenced.Blast-x analysis showed that the four clones were extension like protein,laccase,peroxidase,cell wall-associated hydrolase.Based on a cDNA fragment of laccase,the 1 413 bp cDNA of laccase in Populus simonii × P.nigra was obtained using RT-PCR,including a 1 020 bp open reading frame,and encoding 339 amino acid residues sharing higher homology with other plants.更多还原