【摘要】 目的探讨绝经前后卵巢恶性肿瘤组织中P16、P63蛋白和雌激素受体(ER)表达及临床意义。方法采用免疫组化法检测卵巢恶性肿瘤组织P16、P63和ER的表达,以卵巢良性肿瘤和正常卵巢组织作为对照。结果 P16蛋白在正常卵巢组织、卵巢良性肿瘤和卵巢恶性肿瘤组织中均有表达,但绝经前后卵巢恶性肿瘤组织P16的阳性表达率显著低于卵巢良性肿瘤组织和正常卵巢组织(2χ=5.75~9.64,P<0.05、0.01)。绝经后卵巢恶性肿瘤组织P63蛋白阳性表达率显著高于卵巢良性肿瘤组织和正常卵巢组织(χ2=6.90、8.20,P<0.01),在正常卵巢组织P63蛋白未见表达。绝经后卵巢恶性肿瘤组织ER阳性表达率显著高于卵巢良性肿瘤组织和正常卵巢组织(χ2=14.86、16.68,P<0.01)。卵巢恶性肿瘤组织P16和P63蛋白表达呈负相关(r=-0.277,P<0.05),ER和P16、P63蛋白的表达无相关性(P>0.05)。结论 P16、P63蛋白和ER在绝经后卵巢恶性肿瘤的发生发展中起重要作用,其对指导临床诊断和预后有一定意义。更多还原

【Abstract】 Objective To investigate the expressions of P16,P63 genes and estrogen receptor(ER) and their clinical significance in malignant ovarian tumors(MOT),before and after menopause.Methods The expressions of P16,P63 and ER in MOT tissue were detected by immunohistochemistry,and tissues of benign ovarian tumor(BOT) and normal ovary were used as controls.Results P16 was demonstrated in tissues of normal ovary,BOT and MOT,but its positive expression in MOT was significantly lower than that in tissues of normal ovary and BOT(χ2=5.75-9.64,P<0.05,0.01).In post-menopause MOT,the positive expressions of P63 and ER were markedly higher than that in BOT and normal ovary(χ2=6.90-16.68,P<0.01).No expression of P63 was detected in normal ovarian tissue.In MOT,the expression of P16 was negatively correlated with P63(r=-0.277,P<0.05);no correlation between the expression of ER with P16 and P63 was noted(P>0.05).Conclusion Estrogen receptor,P16 and P63 play an important role in the occurrence and development of post-menopause malignant ovarian tumors,which is of a certain significance in guidance of clinical diagnosis and prediction of prognosis.更多还原