【摘要】 目的研究新藤黄酸(Gambogenic acid,GNA)抑制肺腺癌细胞株A549增殖的作用及其相关机制。方法不同浓度GNA分别作用A549细胞24、48和72 h,甲基噻唑基四唑(methyl thiazolyl tetrazolium,MTT)比色法检测药物对A549细胞增殖的抑制率,吖啶橙/溴化乙啶(acridine orange/ethidium bromide,AO/EB)染色荧光显微镜检测细胞的凋亡;乳酸脱氢酶(lactate dehydrogenase,LDH)法检测GNA对A549细胞损伤作用。结果 MTT法检测结果显示,GNA在0~8μmol/L浓度范围内、24~72 h时间范围内对A549的生长有明显抑制作用,且呈浓度和时间依赖关系;相差显微镜观察表明GNA作用A549细胞48 h后,细胞变圆,呈半贴壁状态;AO/EB染色荧光显微镜观察显示GNA作用48 h后,细胞出现典型的凋亡特征;LDH实验进一步证实GNA在0~4μmol/L浓度范围内对正常细胞未见明显毒性作用。与空白对照组比较,8μmol/L GNA显著降低LDH释放。结论 GNA具有体外抑制肺腺癌细胞株A549增殖的作用,在0~4μmol/L浓度范围内GNA可能是通过诱导细胞凋亡而发挥抑制A549细胞增殖的作用。更多还原

【Abstract】 Objective To investigate anti-proliferation effect of Gambogenic acid(GNA) on lung adenocarcinoma A549 cells and its underlying mechanisms.Methods A549 cells were treated with different concentration of GNA for 24,48 and 72 h,respectively;the inhibitive effect was measured by methyl thiazolyl tetrazolium(MTT) assay;alternation of ultra structure was detected by AO/EB staining under fluorescent microscope;lactate dehydrogenase(LDH) assay was applied to determine whether GNA induced A549 cells necrosis.Results MTT assay evidenced that GNA inhibited A549 cells proliferation in a dosage dependent manner within the concentration ranging from 0 to 8 μmol/L and time-dependent manner from 24 to 72 h;AO/EB staining under fluorescent microscope showed that 24 h GNA treatment could induce typical apoptosis in A549 cells;LDH assay showed that no significant difference in the LDH release was observed after GNA treatment for 24 h in the concentration ranging from 1 to 4 μmol/L,while 8 μmol/L GNA could statistically induce LDH release in comparison with the control group(P<0.01).Conclusion GNA has the capacity to inhibit the proliferation of human lung adenocarcinoma A549 cells.Its underlying mechanisms is related to apoptosis inducing.更多还原