【摘要】 [目的]筛选蛇足石杉中石杉碱甲的酶法提取的最佳提取条件。[方法]采用纤维素酶酶解细胞壁,从而溶出更多的蛇足石杉细胞中的物质,并设计正交试验选出酶法提取的最佳提取条件。[结果]蛇足石杉中石杉碱甲的酶法提取的最佳工艺条件为纤维素酶用量0.125 g,酶解pH4.5,酶解温度60℃,酶解时间2.5 h;在此条件下,蛇足石杉中石杉碱甲的提取率为0.589‰;各因素中温度对纤维素酶的酶解效果影响最大。与酸提法相比,酶法提取的石杉碱甲的提取率提高了40.3%。[结论]该方法简便快捷、提取率高,可用来提取蛇足石杉中石杉碱甲。更多还原

【Abstract】 [Objective] To screen the optimum extraction technology of huperzine A from Huperzia serrata by enzymatic method.[Method] Using cellulase to hydrolyze the cytoderm,orthogonal test was carried out to optimize the extraction technology of huperzine A from Huperzia serrata by enzymatic method.[Result] The optimum extraction technology of huperzine A from Huperzia serrata by enzymatic method was a combination of 0.125 g cellulase,4.5 pH,60 ℃ enzymolysis temperature and 2.5 h enzymolysis time,under which the extraction rate of huperzine A could reach 0.589‰.Compared with the acidsoak method,the extraction rate of huperzine A by enzymatic method increased by 40.3%.Besides,hydrolysis temperature was the biggest influence factor to the extraction of huperzine A from Huperzia serrata by enzymatic method.[Conclusion] This method was simple,convenient and efficient,which could be used for extracting huperzine A from Huperzia serrata.更多还原