【摘要】 目的:解决野生药用植物黄花石蒜外植体大小影响其组织培养效果的难题。方法:选用其鳞茎的带底盘双内层鳞片、带底盘双中层鳞片为材料,采用长宽为9.0×8.0mm、6.0×5.0mm、3.0×2.0mm3种不同大小的外植体,接种在MS+9mg/LBA+5mg/LNAA+0.7%琼脂+3%蔗糖与MS+5mg/LBA+9mg/LNAA+0.7%琼脂+3%蔗糖2种培养基上进行培养,研究了黄花石蒜外植体的不同大小对其组织培养效果的影响。结果与结论:6.0×5.0mm中等大小的带底盘双内层鳞片外植体在MS+9mg/LBA+5mg/LNAA+0.7%琼脂+3%蔗糖的培养基上培养有利于不定芽、不定根的发生,产生褐变量也较少。相关分析表明,不同大小的2种外植体在2种培养基上的培养效果为极显著正相关,这进一步说明,在一种培养基中培养效果较好的外植体大小在另一种培养基中其培养效果仍然较好。6.0×5.0mm中等大小的带底盘双内层鳞片外植体的培养效果较其他外植体好,其次为6.0×5.0mm中等大小的带底盘双中层鳞片外植体。更多还原

【Abstract】 Objective :To solve the puzzle about the right size of the explant of wild medicinal plant Lycoris aurea for tissue culture. Methods :three-size explants:9.0×8.0 mm、6.0×5.0 mm、3.0×2.0 mm of endothecium bulb-scale joined by a strip of stem plate and middle-layer bulb-scale joined by a strip of stem plate were cultured on two kinds of the medium:MS+9 mg/L BA +5 mg/L NAA+0.7% agar +3% cane sugar and MS+5 mg/L BA +9 mg/L NAA+0.7% agar +3% cane sugar,and the culture effects of the three-size explants for Lycoris aurea were studied.Results and Conclusion :The results showed that the explant size on 6.0×5.0 mm of the endothecium bulb-scale cultured on the medium:MS+9 mg/L BA +5 mg/L NAA+0.7% agar +3% cane sugar had the advantage of forming adventitious buds and roots,and inhibiting brown samples,and to the middle-layer bulb-scale explant of the same size cultured on the same medium was next. Correlation analysis showed that the culture effect of the three-size explants on one kind of medium was significantly correlated with that of theirs on another kind of medium,which further proved that the result of Duncan’s multiple range test,i.e. if the effect of an explant size on one kind of medium were better,its effect on another kind of medium would also be better. Both Duncan’s multiple range test and correlation analysis indicated that the effect of the size on 6.0×5.0 mm explant of the double endothecium bulb-scale joined by a strip of stem plate was better than those of the other sizes on the experimental explants,and to that of 6.0×5.0 mm size of the double middle-layer bulb-scale joined by a strip of stem plate was next.更多还原