【摘要】 目的探讨人参皂苷Rg3对人成纤维细胞增殖及凋亡的影响。方法自瘢痕疙瘩患者手术切取的瘢痕组织分离、培养得到成纤维细胞。收集处于指数生长期的成纤维细胞接种于96孔板,加入GS-Rg3,终浓度为25、50、100、200μg/ml,对照组加入等量的培养液,放入37℃、5%CO2、孵箱内分别培养24、48、72 h。用MTT法计算抑制率。应用流式细胞术进行测定,记录细胞周期各时相以及AP区亚峰细胞百分率。结果 GS-Rg3对体外成纤维细胞的抑制率随浓度和时间的增加而增强,最大抑制率为92.8%,最佳抑制浓度为100μg/ml。结论 GS-Rg3对人成纤维细胞增殖有明显的抑制作用,并可诱导其凋亡。更多还原

【Abstract】 Objective To investigate the effects of Ginsenoside-Rg3(GS-Rg3) on human fibroblasts’ proliferation and apoptosis.Methods After isolation and culture,fibroblasts were obtained from patients’s keloids.We collected and planted the fibroblasts in exponential growth phase on 96-hole plates,the added GS-Rg3 with final concentration of 25、50、100、200 μg/ml.Equal Volume medium was added in control groups as well.The plates were cultured in the same incubator with 37℃ and 5% CO2 for 24,48 and 72 hours.Inhibition ratio was calculated ith MTT essay.Cells percentage in different phase of different cell cycle and AP region inferior hump was detected by fluorescence activated cell sorter.Results Inhibition ratio of GS-Rg3 on human extraorgan fibroblasts has positive correlation with concentration and time.The highest inhibition ration is 91.45% while the best concentration is 100 μg/ml.Conclusion GS-Rg3 can inhibit human fibroblasts from proliferation strongly,and induce them to apoptosis as well.更多还原