【摘要】 为研究黄芩有效成分的含量与配比方式在地区间的分异规律,并得出中国野生黄芩的主要分布类型,本研究在全国实地采样,用HPLC分析了35个样地共353份野生黄芩样本的4种主要有效成分的含量,并以测得的4种主要有效成分的含量与配比方式为指标,应用聚类分析和方差分析,对我国野生黄芩进行对比分析和分类研究。结果显示:①黄芩有效成分的含量与生长环境有密切关系,我国野生黄芩的主要有效成分含量存在地域差异,地理环境接近的区域生长的黄芩质量比较相似;②就黄芩的主要有效成分而言,我国的野生黄芩可分为二大类,其中C1型黄芩的有效成分含量低,主要分布在冀北山地、辽东低山丘陵、内蒙古地区和东北地区,C2型黄芩的有效成分含量高,主要分布区包括黄土高原区和西辽河平原。本文研究成果可为今后选用不同地区的黄芩药材提供依据。更多还原

【Abstract】 To reveal regional differences in the contents and ratios of major active components in wild Scutellaria baicalensis G,a traditional Chinese herb medicine,and to summarize major types of wild S.baicalensis in China,a serial of studies were conducted in the years 2007 and 2008.In August and September of 2007 and 2008,a total of 353 individuals of S.baicalensis herbs,gathered from 35 different sampling plots scattered all over the herb’s national distribution areas in China,were picked up for measuring the component contents.The contents of active pharmic components in the herbal samples,i.e.,baicalin,wogonoside,baicalein,and wogonin,were measured by the HPLC method.On the basis of the average contents and ratios of 4 major active components in samples,cluster analysis(CA)and analysis of variance(ANOVA)were applied to determine spatial differences in the samples with respect to their regional characteristics among wild S.baicalensis sampled from different provinces in China.It can be concluded that:1)by comparing their average contents of major active components,a regional difference clearly exists among different S.baicalensis samples in China,and the contents of the major active components of S.baicalensis from different sample sources are closely related to the environmental conditions of their areal origins.This makes the herbs from close origins have similar component contents and vice versa;2)the authors generally classified the wild S.baicalensis G.in China into 4 distribution types through field sampling conducted for determination of the active contents in the samples by the HPLC,CA as well as ANOVA.Given statistical significance and representativeness of samples,the samples of the wild S.baicalensis in China can finally be grouped into 2 primary distribution types using the content of active pharmic components as indicators:Class I and Class II.Class I has a lower level of the contents of major active components,mainly located in the northern mountainous areas of Hebei,the low mountains and hills in eastern Liaoning,Inner Mongolia and northeastern China.Class II has a relatively higher concentration on component contents,primarily distributed in the Loess Plateau in northern China and the plain of the Xiliao River.These findings could be taken as a basis for choosing the genuine and typical S.baicalensis herbs from different regions.更多还原