【摘要】 【目的】探究茉莉酸(Jasmonic acid,JA)诱导的钙动员的来源及钙调素(CaM)在JA信号通路中的作用。【方法】以拟南芥(Arabidopsis thaliana columbia)为材料,采用不同类型的离子通透性通道抑制剂(或拮抗剂)预处理拟南芥叶肉细胞,利用激光共聚焦显微镜检测其对JA诱导的[Ca2+]cyt升高的影响;同时还利用上述拮抗剂、钙调素(CaM)拮抗剂处理10d的拟南芥幼苗,采用Northern blot方法检测了其对JA诱导的VSP表达的影响。【结果】胞内及胞外钙离子共同参与JA诱导的钙动员,JA诱导的Ca2+通过CaM或CaM相关蛋白进一步传递JA信号。【结论】质外体钙离子的流入和胞内钙库中钙离子的释放均参与JA诱导的钙离子动员,Ca2+可能通过CaM或CaM相关蛋白传递JA信号,进一步诱导JA反应基因的表达。更多还原

【Abstract】 Objective The objective of this experiment is to evaluate the role of intracellular and extracellular Ca2+ and calmodulin (CaM) in JA signaling.Method The Laser Scanning Microscopy was used to detect the changes of [Ca2+]cyt of Arabidopsis thaliana leaf cells which pretreated with different types of calcium channel blocker.Moreover,the expression of JA response gene,VSP,was also investigated after pretreated with the above blocker and antagonist of CaM.Result The results showed that extracellular and intracellular calcium both involved in the JA induced Ca2+ mobilization,and then Ca2+ exerts its functions through activating the CaM or CaM related proteins.Conclusion The apoplast calcium influx and the calcium release from the calcium stores are both involved in the JA induced calcium mobilization,then the JA induced Ca2+ transmits the JA signal through CaM or CaM related proteins,and regulate the JA responsive genes.更多还原