【摘要】 运用Nested-PCR技术对来自广西部分市、县的40个黄皮和九里香进行柑桔黄龙病(CitrusHuanglongbing,HLB)病原检测,结果表明:从部分黄皮和九里香样品抽提的DNA中,可扩增出一条特异性的400bp条带,说明广西种植的黄皮和九里香均可感染黄龙病;在柑桔黄龙病疫区,黄皮和九里香黄龙病感染率分别为35.0%和40.0%,黄龙病感染率已相当高。为了减少柑桔黄龙病的发生,有利于对柑桔黄龙病进行综合防控,不宜在柑桔种植区栽种黄皮和九里香,铲除已感染黄龙病的植株。更多还原

【Abstract】 Nested-PCR was used for the detection of the pathogen of citrus Huanglongbing (HLB) on 40 samples ofClausena lansium andMurraya paniculata from several cities of Guangxi. The result demonstrated that a specific 400 bp band could be amplified from part of the DNA extracted from these plant materials, respectively. It indicated that Clausena lansium and Murraya paniculata from Guangxi could be infected by HLB. In HLB-infected area, HLB infection rates ofClausena lansium andMurraya paniculata were 35.0% and 40.0% , respectively. For the purpose of reduction of HLB infection rates and integrated control of HLB, Clausena lansium andMurraya paniculata should not be planted in the citrus planting area, and plants infected by HLB should be removed as soon as possible.更多还原