【摘要】 采用略加修改的NLN培养基(无机大量元素1/2)和甘蓝型油菜小孢子的胚状体诱导程序,培养了5个中国菜用小白菜品种和1个中国大白菜品种的小孢子,进行了双单倍体的诱导。结果表明,不同品种的小孢子产胚量差异很大,在接种的6个基因型中,有5个诱导出胚,诱导率83.33%。其中的中萁青抗热605产胚量最高,是8.68个胚/10蕾,最低的是鸡冠菜,其产胚率是0.42个胚/10蕾。这些胚在添加了NAA和PP333(多效唑)的B5培养基发育成健壮的小植株,已移栽到田间。关于6个基因型的小孢子胚胎发生能力,还有待进一步研究。更多还原

【Abstract】 The microspores of 5 genotypes of Brassica campestris L.ssp.chinensis and 1 genotype of Brassica campestris L.ssp.pekinensis were cultured in vitro using modified NLN medium (1/2 of inorganic major element) and the procedure of microspore culture of Brassica napus.The results showed that the embryo yield between genotypes was obviously different.The embryos regenerated from the microsores of 5 out of 6 genotypes with 83.33% of tested materials.The embryo yield of ‘ Zhongqiqingkangre605 ’ was the highest with 8.68 embryos per 10 buds and jiguancai was the lowest with 0.42 embryos per 10 buds.All embryos produced from the microspores of 5 genotypes developed into stronger plantlets on B5 medium added NAA and PP333,and then transplanted to the field.The embryogenesis capacity of microspores of 6 genotypes needs to be further studied.更多还原