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PEG-6000模拟干旱对水稻苗期根系形态和部分生理指标影响的研究

Study on Effect of Drought Stress with PEG-6000 on Root Morphology and Some Physiological Indices in Rice Seedling

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【作者】 马廷臣余蓉蓉陈荣军曾汉来张端品

【Author】 Ma Tingchen1,2,3,Yu Rongrong3,Chen Rongjun3,Zeng Hanlai2,Zhang Duanpin2(1Rice Research Institute;Anhui Academy of Agricultural Sciences,Key Laboratory of Rice Genetics and Breeding of Anhui Province Hefei,Branch of National Rice Improvement Center;Hefei 230031;2National Key Laboratory of Crop Genetic Improvement,Huazhong Agricultural University,Wuhan 430070;3Institute of Subtropical Agriculture,The Chinese Academy of Sciences,Changsha410125)

【机构】 安徽省农业科学院水稻研究所安徽省水稻遗传育种重点实验室国家水稻改良中心合肥分中心华中农业大学作物遗传改良国家重点实验室中国科学院亚热带农业生态研究所

【摘要】 随着全球气候变化,干旱对农作物的产量的影响越来越大,植物地上部分对干旱逆境的响应的研究较多,地下根系在干旱逆境时的变化研究较少,笔者研究了不同PEG浓度处理对2个不同抗逆性的水稻品种根体积、根粗、最长根长3个形态指标和SOD活性、MDA含量、Pro含量、根系活力4个生理指标的影响。结果如下:耐旱品种和敏感品种根部的形态指标都受严重干旱(T3处理)抑制,敏感品种更为明显;形态指标在T0、T1、T2、T3处理下随着处理时间的增加而增大,不同浓度处理之间一般处理3~4天后才会产生明显差异;CAT活性和SOD活性随处理浓度增加而增大,随处理时间延长,抗旱品种SOD的活性增加的速度快于敏感的品种,下降的速度慢于敏感品种;MDA含量和Pro含量随着处理浓度升高而增加,随处理时间增加而增大,敏感品种高于耐旱品种,作为抗旱选择指标并不合适,作为胁迫敏感指标更合适;根系活力随处理时间增加而增大,其最适的处理浓度为T1;相关分析表明根系活力与形态指标正相关极显著,根粗、CAT活性和SOD活性与MDA含量和Pro含量负相关显著或极显著,MDA含量和Pro为极显著的正相关关系,CAT活性和SOD活性也为极显著的正相关关系。

【Abstract】 With the change of climate,more and more effect of drought stress on plant production.Two different drought resistant varieties had been treated by PEG6000 grades(T0,T1,T2 and T3) in rice seedling.Their morphology and physiological indices were surveyed and concluded as followed:The morphology indices of 2 varieties were restrained by strictness drought(T3 treatment) and the sensitive one was more effective than the resistant one.The morphology indiced augment with treatment time prolonging by all PEG6000 grades and their difference appeared after 34 days.CAT and SOD activity augmented with treatment time prolonging and PEG6000 concentration increasing.The resistant one augments faster than the sensitive one and descends slower than the sensitive variety.MDA and Pro content augmented with treatment time prolonging and PEG6000 concentration increasing.The effect of the sensitive variety was stronger than the resistant one.So they were not adapted to be drought resistant indices.Root activity augmented with treatment time prolonging.The consistent with or coincide with or et al optimum treatment was T1.Correlation analysis showed that there were very significantly positive correlations between root activity and morphology indices,and significantly or very significantly negative correlations between root diameter,CAT activity,SOD activity and MDA content,praline content.MDA content was very significantly positively related to praline content,CAT activity was very significantly positive correlation to SOD activity.

【关键词】 PEG-6000水稻根系形态生理指标
【Key words】 PEG-6000riceroot systemmorphology and physiological indices
【基金】 国家自然科学基金项目(30571148、30671273);安徽省国际合作项目(08080703030);安徽省外国专家局引智项目(20083400089)
  • 【文献出处】 中国农学通报 ,Chinese Agricultural Science Bulletin , 编辑部邮箱 ,2010年08期
  • 【分类号】S511.01
  • 【被引频次】82
  • 【下载频次】1141
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