【摘要】 目的对光活化α-三噻吩(α-T)引起的蚊幼虫脂质过氧化进行组织化学定位研究。方法用冷Schiff组织化学法,检测光活化α-T处理后的白纹伊蚊幼虫,光学显微镜下与对照组比较,观察光活化α-T引起的脂质过氧化组织定位。结果对照组组织均未着色,实验组中肠组织细胞、围食膜、马氏管刷状缘及管腔出现紫红色着色。结论光活化α-T能使白纹伊蚊幼虫的组织发生脂质过氧化,且脂质过氧化的主要组织靶标为中肠和马氏管。更多还原

【Abstract】 Objective To histochemically locate the lipid peroxidation induced by photosensitized α-terthienyl(α-T) in Aedes albopictus larvae.Methods Cold-schiff was employed to observe the Ae.albopictus larvae exposed to photosensitized α-T under optical microscope in comparison to the control group to evaluate the histochemical localization of lipid peroxidation.Results Tissues in the control group remaining unstained,the midgut epithelium,the peritrophic membrane,the brush border and the lumen of malpighian tubules were stained purple in the experimental group.Conclusion Photosensitized α-T could induce lipid peroxidation in tissues of Ae.albopictus larvae,involving primarily the midgut and malpighian tubules.更多还原