【摘要】 目的检测系统性红斑狼疮(SLE)患者人白细胞抗原G(HLA-G)14bp插入/缺失(ins/del)多态性和血浆可溶性HLA-G(sHLA-G)水平,并分析它们与SLE的关联性。方法聚合酶链式反应(PCR)检测231例SLE患者及367名健康体检者的HLA-G 14bp ins/del多态性的基因型分布,酶联免疫吸附法(ELISA)检测其中96例SLE患者及74名健康体检者血浆sHLA-G水平。结果HLA-G14bp ins/del多态性位点和基因型频率分布在正常人组和SLE组均无统计学意义(P均>0.05),血浆sHLA-G水平在SLE患者组为(230.2±192.2)U/ml,明显高于正常人组的(118.3±38.1)U/ml(P=0.0001);血浆sHLA-G水平增高的SLE患者易出现中枢神经系统受累(P=0.007),且较血浆sHLA-G水平正常的患者病情更重(P=0.027)。进一步分析血浆sHLA-G水平增高和正常SLE患者的HLA-G 14bp ins/del多态性位点和基因型分布,未发现两组间有差异(P=0.675)。结论血浆sHLA-G水平在SLE患者中明显增高提示sHLA-G可能参与到SLE发病机制中,其水平高低主要取决于SLE病情严重性及活动性,而与HLA-G1 4bp ins/del多态性无关。更多还原

【Abstract】 Objective To determine the association of HLA-G 14 bp insertion/deletion polymorphism and plasma soluble HLA-G(sHLA-G)levels with systemic lupus erythematosus(SLE).Methods Polymerase chain reaction(PCR)was performed to detect the genotypes of HLA-G 14 bp ins/del polymorphism in 231 SLE patients and 367 healthy controls,and soluble HLA-G(sHLA-G)levels were determined by enzyme-linked immunosorbent assay in 96 SLE patients and 74 healthy controls.Results No significant differences were found in HLA-G 14 bp ins/del allelic frequencies and genotype distributions between SLE patients group and the control group(P>0.05).However,plasma concentration of sHLA-G was significant higher in SLE patients than that in healthy controls(230.2±192.2)U/ml vs.(118.3±38.1)U/ml,P=0.0001).Moreover,the patients with increased levels of sHLA-G had higher incidence of central nervous system involvement(P=0.007)and more severe disease activity(P=0.027)in comparison with patients with normal plasma sHLA-G levels.Finally,analysis of HLA-G 14 bp ins/del polymorphism in patients with increased sHLA-G levels revealed that there was not different from that in patients with normal sHLA-G levels(P=0.675).Conclusions The increased production of sHLA-G indicates that sHLA-G may play an important role in the pathogenesis of SLE,the expression of sHLA-G may be associated with disease activity and severity of lupus patients,but be independence of HLA-G 14 bp ins/del polymorphism.更多还原