【摘要】 在蛋白质晶体衍射数据采集过程中样品会受到X射线的辐射损伤.辐射损伤打乱分子中的共价键造成蛋白质分子中二硫键的断裂和错位,甚至打乱晶格结构,是引起蛋白质晶体学中采集准确衍射数据误差的一个主要原因.通过对溶菌酶蛋白及Acutolysin-C蛋白单波长反常散射数据的分析,讨论了样品在受辐照过程中累积的辐射损伤对衍射数据以及反常散射信号的影响.更多还原

【Abstract】 Samples suffer from X-ray radiation damage during the protein crystal diffraction data collection process.Radiation damage disrupts covalent bonds in molecules,causing breakage and delocalization of disulphide bonds,even disrupting the lattice structure,and is one of the major reasons for errors occurring during the collection of accurate diffraction data in protein crystallography.Through analyzing the single-wavelength anomalous diffraction(SAD)data of lysozyme and Acutolysin-C protein,the impact of radiation damage accumulated on samples in the exposure process on the diffraction data and on the signal of anomalous diffraction was discussed.更多还原