【摘要】 目的:通过观察胞质型磷脂酶A2(cPLA2)和环氧化酶-2(COX-2)蛋白在正常口腔黏膜和口腔不典型增生中的表达情况,研究cPLA2和COX-2在口腔不典型增生发生过程中的作用。方法:收集了9例正常口腔黏膜、36例口腔黏膜不典型增生标本,制作石蜡标本并行cPLA2和COX-2的免疫组化染色。结果:cPLA2和COX-2在口腔不典型增生的表达明显高于在口腔正常黏膜中的表达(P<0.01);cPLA2和COX-2在轻度不典型增生中的表达明显低于在中度和重度不典型增生中的表达(P均<0.01);cPLA2和COX-2的表达呈显著相关性(P<0.01)。结论:cPLA2和COX-2在口腔黏膜不典型增生发生过程中具有明显的协同作用,提示cPLA2可以作为口腔不典型增生化学治疗的新的分子靶标。更多还原

【Abstract】 Objective: To investigate the expression of cytosolic phospholipase A2(cPLA2) and cyclooxygenase 2(COX-2) in normal oral mucosa and dysplastic oral mucosa.Methods: Nine tissue samples of normal oral epithelium,36 cases of oral dysplasia were collected.The expressions of cPLA2 and COX-2 in these tissues were analyzed by Immuno-histological chemistry.Results: cPLA2 and COX-2 were expressed much higher in oral dysplasia(P<0.01).Among the oral dysplasia cases,cPLA2 expression in mild dysplasia was found to be significantly lower than in moderate and severe dysplasia(P<0.01).The pattern of staining of COX-2 was similar to cPLA2 in normal oral epithelia and oral dysplasias.There was no statistically significant relationship established between the COX-2 expression and the pathological grade assessed to the oral dysplasia.Within each sample,a significant correlation was revealed between cPLA2 expression and COX-2 expression(P<0.01).Conclusion: cPLA2 and COX-2,key factors in PGE2 biosynthesis,worked in concern to promote the progression of normal oral epithelium toward oral dysplasia.更多还原