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Mg2+和Fe3+对日本血吸虫毛蚴的趋向作用观察及在“模拟钉螺”中的应用

The effect of Mg2+ and Fe3+ on the distribution of Schistosoma japonicum miracidia and use of this information in simulated snails

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【作者】 陈喜珪沈定文覃金红陈鹏程石磊刘洋卢恒聪何昌浩

【Author】 CHEN Xi-gui1,SHEN Ding-wen1,QIN Jin-hong1,CHEN Peng-cheng1,SHI Lei2,LIU Yang2,LU Heng-cong2,HE Chang-hao2 (1.Basic Medical College of Xianning College,Xianning 437100,Hubei,China;2.Department of Pathogenic Biology,Tongji Medical College,Huazhong University of Science and Technology)

【机构】 咸宁学院基础医学院华中科技大学同济医学院病原生物学系

【摘要】 目的观察Mg2+、Fe3+对日本血吸虫毛蚴的趋向作用及其在"模拟钉螺"中的应用。方法采用改良Ro-berts比较法。结果加入1.00mol/L的Mg2+溶液时,实验组和对照组中日本血吸虫毛蚴的数目比为93和21(P<0.01);Fe3+浓度为0.05、0.10、0.25、0.50和1.00mol/L时,实验组和对照组中日本血吸虫毛蚴的数目比分别是8l和29(P<0.01)、24和5(P<0.01)、225和39(P<0.01)、41和7(P<0.01)、52和17(P<0.01)。一定配比的明胶混合物释放出的Fe3+吸引日本血吸虫毛蚴的能力强于钉螺处理过的水。结论适宜浓度的Mg2+和Fe3+能显著影响日本血吸虫毛蚴的趋向运动,"模拟钉螺"吸引日本血吸虫毛蚴的能力强于钉螺处理过的水。

【Abstract】 Objective To observe the effect of Mg2+ and Fe3+ on the distribution of Schistosoma japonicum miracidia and use this information in simulated snails.Methods A modified form of Roberts’ method of comparison was used.Results The ratio of S.japonicum miracidia for the experimental group and control group was 93:21(P<0.01) when adding 1.00 mol/L Mg2+.The ratio of S.japonicum miracidia for the experimental group and control group was respectively 81:29(P<0.01),24:5(P<0.01),225:39(P<0.01),41:7(P<0.01),and 52:17(P<0.01) when the concentration of Fe3+ was 0.05,0.10,0.25,0.50,and 1.00 mol/L.Fe3+ released from a gelatin mixture with a certain proportion of Mg2+ and Fe3+ had greater ability to attract S.japonicum miracidia than water conditioned by snails.Conclusion Mg2+ and Fe3+ significantly affected the distribution of S.japonicum miracidia.Simulated snails had greater ability to attract S.japonicum miracidia than water conditioned by snails.

【基金】 湖北省教育厅自然科学基金资助项目(No.D200628009)
  • 【文献出处】 中国病原生物学杂志 ,Journal of Pathogen Biology , 编辑部邮箱 ,2010年07期
  • 【分类号】R383.24
  • 【被引频次】3
  • 【下载频次】59
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