【摘要】 目的对上海市2008～2009年手足口病(Hand,Footand Mouth Disease:HFMD)部分病例,进行病原学与分子流行病学研究。方法用人横纹肌肉瘤(Human Rhabdomyosarcoma,RD)细胞和非洲绿猴肾(African Green Monkey Kidney,Vero)细胞,对采集到的HFMD病例临床标本进行病毒分离,用逆转录-聚合酶链反应(Reverse Transcription-Polymerase Chain Reaction,RT-PCR)、序列测定和同源性分析的方法,对分离到的病毒进行分析鉴定。结果上海市2008～2009年分别从66.5%(105例)和69.0%(20例)的患者中分离到肠道病毒(Enterovirus,EV),分离到的EV71型(EV71)属于C4基因亚型,但与上海市2002年EV71分离株相比已发生了较大的变异。分离到的柯萨奇病毒A组16型(Coxsackievirus Group A Type16,CA16)属于B基因型,分成2个明显的分支,每个分支内部都有近两年的病毒共存,且与上海市2002年CA16分离株相比变异不大。结论 2008～2009年导致上海市HFMD流行的病毒仍以EV71和CA16为主;在采用RD和Vero两种细胞同时分离病毒时,不同类型标本的病毒分离阳性率差异无统计学意义。更多还原

【Abstract】 Objective To study etiology and molecular epidemiology of the parts of the hand foot and mouth disease cases in Shanghai,2008-2009.Methods Cell culture was performed to all clinical specimens from cases with RD and Vero cell lineage.RT-PCR,sequencing and phylogenetic analysis were implemented to identify serotypes of virus isolates and made molecular epidemiology analysis.Results Enteroviruses were isolated from 66.5%(105 cases) and 69.0%(20 cases) respectively from cases of 2008-2009.EV71 isolates were still C4 subgenotype,but genetic diversity was found compared with EV71 isolates of Shanghai in 2002.CA16 isolates were B genotype with two clusters,which showed that different transmission chains were coexist.Conclusions EV71 and CA16 were the major agents that caused the epidemic of hand foot and mouth disease in recent two years.No significant difference was found for positive rates between different kinds of clinical specimens when RD and Vero cell lineage were used.更多还原