【摘要】 目的:通过反义核酸技术抑制miR-20、miR-106的表达,检测对293T细胞增殖的影响。方法:应用特异性针对miR-20、miR-106的反义核酸转染293T细胞,用形态学、流式细胞技术研究反义核酸对细胞增殖的抑制作用,用定量PCR和ELISA研究miRNA和相关靶基因的表达。结果:研究发现反义核酸可有效抑制miR-20和miR-106的表达,并明显抑制293T细胞的增殖;研究还表明反义miR-106可明显上调抑癌基因Rb的表达。结论:反义核酸通过抑制miRNA表达,上调抑癌基因Rb的表达,能明显抑制293T细胞增殖。更多还原

【Abstract】 AIM: To inhibit 293T cell proliferation by reducing miR-20 and miR-106 expression with antisense RNA. METHODS: Antisense RNA ( or antisense oligonucleotides,ASO) specific to miR-20 and miR-106 was synthesized,and the 293T cells were treated with these antisense RNA. Then,the cell proliferation derived from suppression of antisense RNA was studied by microscopy and fluorescence-activated cell sorter. The expression of miRNA and its target gene Rb were analyzed by real-time PCR and ELISA,respectively. RESULTS: Our results showed that ASO could inhibit the expression of miR-20 and miR-106 effectively and inhibit 293T cell proliferation. The results also demonstrated that ASO specific to miR-106 could up-regulate anti-oncogene Rb expression. CONCLUSION: ASO could inhibit 293T cell proliferation by inhibiting miRNA expression and up-regulating Rb expression.更多还原