【摘要】 目的评价免疫组化、明胶酶谱、Western blot检测涎腺良恶性肿瘤中基质金属蛋白酶MMP-2、MMP-9表达的优点与局限性。方法分别运用免疫组化、明胶酶谱、Western blot对34例涎腺良恶性肿瘤进行MMP-2、MMP-9定位、半定量、定量检测和活性酶含量的分析,比较各方法检测结果的差异。结果免疫组化方法能定位、半定量检测出基质金属蛋白酶在良恶性肿瘤间的差异;Western blot能定量说明基质金属蛋白酶在良恶性肿瘤间的差异;明胶酶谱法能检测出MMP-2、MMP-9酶原和活性酶在良恶性肿瘤间的差异。结论免疫组化、Western blot、明胶酶谱三种方法结合,既发挥免疫组化细胞定位的优势,又克服了免疫组化法非精确定量的弱点,并打破其不能辨别酶原与活性酶的局限性,更加客观准确反映各型肿瘤组织中MMP-2、MMP-9的实际表达。更多还原

【Abstract】 Objective To compare the merits and limitations of immunohistochemistry,Gelatin zymography and Western blot analysis in the detection of protein expression of MMP-2 and MMP-9 in salivary gland tumors.Methods Immunohistochemistry,Gelatin zymography and Western blot analysis were used in 8 malignant and 26 benign salivary gland tumors to carry out localization,semi-quantitative detection,quantitative detection and enzyme activity analysis for MMP-2 and MMP-9.Results Immunohistochemical method could locate and semi-quantitative detect the difference between matrix metalloproteinases in benign and malignant tumors.Western blot could quantitative describe the difference between matrix metalloproteinases in benign and malignant tumors.Zymography could detect differences between zymogen and active enzyme of MMP-2 and MMP-9 in benign and malignant tumors.Conclusion The combination of immunohistochemistry,Gelatin zymography and Western blot analysis can not only play the advantage of cellular localization by immunohistochemistry alone,but also overcome the weaknesses of inexact quantitative by immunohistochemistry and the limitations of non discernment of proenzyme and active enzyme,so as to reflect the actual expression of MMP-2 and MMP-9 in various types of tumor tissue more objectively and accurately.更多还原