【摘要】 目的:观察人胎盘组织液抗大鼠腹膜粘连作用并探讨其作用机制。方法:采用大鼠腹膜缺损/盲肠刮伤模型,设立模型组、假手术组和人胎盘组织液组,于术后7、14d麻醉下再次开腹,肉眼评定盲肠与周围组织的粘连情况,并取粘连组织进行组织病理学观察,包括HE染色、Masson染色、免疫组化TGF-β1测定。结果:与模型组相比,人胎盘组织液组粘连评分明显降低(P<0.05)。HE染色和Masson染色显示人胎盘组织液组肉芽组织量少,肉芽组织疏松,炎细胞明显减少,胶原纤维明显减少。免疫组化结果显示人胎盘组织液抑制了TGF-β1的表达。结论:人胎盘组织液通过抑制TGF-β1表达,抑制成纤维细胞的增生和胶原蛋白的形成,减轻了大鼠术后粘连发生的程度和范围。更多还原

【Abstract】 Objective:To observe the efficacy of human placenta tissue hydrolysate on prevention experimental postoperative peritoneal adhesions and discuss its mechanism of action.Methods:A rat model was created by abdominal wall defect and cecal abrasion was used,there were three groups,including model group,model with human placenta tissue hydrolysate group and sham-o control group.Postoperative 7 days and 14 days,the adhesion of cecum and the adjacent tissues were evaluated by naked eyes,then the adhesion tissues were selected to perform histopathological observation,including HE staining,Masson’s staining,and TGF-β1 expression was determined by immunocytochemical staining.Results:Compared with model group,the model with injection of human placenta tissue hydrolysate significantly reduced in adhesions score(P<0.05),the studies by HE and masson’s staining showed that the adhesion tissue between theparietal peritoneum and cecum had less inflammation cells,less fibroblast and less collagen than model group.TGF-β1 expression had been suppressed in the model with human placenta tissue hydrolysate by immunocytochemical staining.Conclusion:Human placenta tissue hydrolysate can reduce TGF-β1 expression and restrain the inflammation cells,fibroblast cells and collagen to prevent the experimental postoperative peritoneal adhesions.更多还原