【摘要】 采用酚-硫酸法测定木素磺酸铵中糖含量,以葡萄糖为对照,苯酚-硫酸作显色剂,用紫外分光光度计在490 nm处测定吸光度。结果显示:葡萄糖浓度为20～120μg/mL时线性关系显著,R2=0.995 9;精密度RSD=0.034%,稳定性RSD=0.102%,回收率98.99%,RSD=0.871%,重现性RSD=0.336%。酚-硫酸法测定木素磺酸铵中糖含量简便易行,精密度、稳定性、回收性、重现性良好。更多还原

【Abstract】 Taking glucose as CK and phenol sulfuric acid as chromogenic reagents,the saccharide content in ammonium lignin sulphonate was determined by means of phenol sulfuric method with UV spectrophotometer in 490 nm section.The results showed that there was a significant linear correlation between the glucose content and the photometric value when the glucose concentration was in the range of 20～120 μg/mL,the coefficient R2 was 0.995 9,with precision RSD as 0.034%,stability RSD as 0.102%,recovery rate as 98.99%,recovery RSD as 0.871%,and the repetition RSD as 0.336%.It was indicated that the phenol sulfuric acid method for determining saccharide content in ammonium lignin sulphonate was a way characterized with simplicity,practicality,high precision,good stability and repetition with high recovery rate.更多还原