【摘要】 以15NH4Cl为起始原料,通过改进的Gabriel方法得到S-苄基-D,L-半胱氨酸,再经酶法拆分和解保护后得到15N标记L-胱氨酸。对影响各步反应收率的主要因素进行了考察,在较优的工艺条件下,以15NH4Cl计,15N标记L-胱氨酸收率为13.5%,产物经HPLC、红外、质谱和元素分析等证实,质谱分析表明未出现同位素丰度稀释现象。更多还原

【Abstract】 By using 15NH4Cl as starting material,an improved Gabriel preparation method was used for the synthesis of S-benzyl-D,L-cysteine,which was further converted to S-benzyl-L-cysteine by optical resolution in the presence of aminoacylase.After the removal of protective group,the target compound 15N-L-cystine was obtained.The main controlling factors of the reactions were investigated.Under the optimized conditions,the total yield of L-cystine reached 13.5% based on 15NH4Cl consumed.The product was characterized by HPLC,IR,MS-EI and elemental analysis.The dilution of enrichment was not found in the process.更多还原