【摘要】 目的:研究肝细胞癌(HCC)患者外周血CD95和OX40L mRNA的表达情况,并探讨其临床意义。方法:将研究对象的淋巴细胞与相应荧光标记的单克隆抗体反应,经洗涤、固定后,应用双色流式细胞术分析T淋巴细胞表面CD95+CD3+的表达量。采用荧光定量逆转录聚合酶链反应(FQ-RT-PCR)方法检测外周血单个核细胞共刺激分子OX40L mRNA的表达水平。结果:HCC患者外周血CD95+CD3+的表达水平明显高于健康对照组,差异有统计学意义([34±20)%vs(20±7)%,t=2.960,P<0.01];HCC患者外周血单个核细胞OX40L mRNA表达水平明显低于健康对照组,差异有统计学意义(0.46±0.36vs0.86±0.70,t=2.302,P<0.05)。结论:HCC患者外周血CD95及OX40L分子的异常表达在HCC的发生中起重要作用。更多还原

【Abstract】 Objective: To assess the expression levels of CD95 and OX40 ligand (OX40L) messenger RNA (mRNA) in hepatocellular carcinoma (HCC), and their clinical values thereof. Methods: The lymphocytes of research objects were mixed with corresponding fluorescence labeled monoclonal antibody (McAb); CD95 expression by CD3 positive T cells was quantitatively measured by dual color flow cytometry. The expression of OX40L mRNA was detected in peripheral blood mononuclear cells by fluorescence quantitative reverse transcription polymerase chain reaction(FQ-RT-PCR). Results: The CD95 expression by CD3 positive T cells was significantly higher in patients with HCC (34±20)% compared with that of normal controls (20±7)%(t = 2.960,P < 0.01). The expression level of OX40L mRNA was significantly decreased in patients with HCC compared with that of normal controls (t = 2.302,P < 0.05). Conclusion: These results suggest that abnormal expressions of CD95 and OX40L play crucial roles in peripheral blood of HCC patients at the stage of human hepatocarcinogenesis.更多还原