【摘要】 SSR也称作微卫星DNA,是一种广泛应用的分子标记技术。SSR具有丰富的多态性,基于这种多态性,可以对植物基因组进行分析筛选;SSR的检测方法快速、技术简便。目前,这项技术已用于多种植物的基因组研究,如基因定位及遗传图谱的建立、数量性状标记、杂种优势的利用等。在一些重要的农作物中,已定为了丰富的SSR位点。随着技术的发展,基于SSR技术的新的标记方法不断出现,如:ISSR、RAMP、REMAP。概述了SSR标记技术的原理和特点;介绍了与实验相关的技术和方法;着重整理了近年来在SSR技术上发展起来的新的标记方法;探讨了SSR在植物基因组研究中的应用。更多还原

【Abstract】 One of the markers widely applied in breeding programs is the SSR marker,also called microsatellite.SSRs have been reported to be highly polymorphic in plants and hence highly informative in plants and can be analyzed by a rapid,technically simple and inexpensive PCR-based assay.SSR markers can be used to analyze and filter the plant genome.Now,it had been applied to different plants genome researches.A large number of SSR loci has been genetically mapped in several agronomically important plant species.In this paper,the theory and characteristics was briefly summarized;its experimentation methods and development were presented and the application in plant genome research was discussed.更多还原