【摘要】 目的:观察青藤碱对大鼠肝脏缺血再灌注损伤的影响,探讨其保护大鼠肝脏缺血再灌注损伤作用的机制。方法:通过建立大鼠全肝缺血再灌注损伤模型,应用硝酸酶还原法测定肝脏缺血再灌注后60min血清NO水平变化;测定再灌注60min后肝组织内MDA和SOD含量变化;再灌注60min取肝组织完成肝组织显微结构的观察。结果:肝脏缺血再灌注损伤后血清NO水平降低;青藤碱能提高再灌注后血清NO水平,且能改善肝脏缺血再灌注损伤的微循环,减轻肝细胞内超微结构的损害程度。结论:青藤碱对大鼠肝脏缺血再灌注损伤有保护作用,其主要作用机制是清除氧自由基和改善微循环。更多还原

【Abstract】 Objective:To investigate the protective effects of sinomenine on hepatic injury during ischemia/reperfusion proce-dure, and to investigate the posssible mechanism.Methods:40 SD rats were randomly divided into 4 groups (n=10) :control group, I/R group, sinomenine group 1 (50mg/kg) , sinomenine group 2 (100mg/kg).Hepatic ischemia/reperfusion models were established.At the end of reperfusion, the NO, the SOD and MDA of each group were detected.Results:In sinomenine groups the NO was higherthan that in I/R group (P<0.01).The SOD of hepatic tissue was higherin sinomenine groups than that in I/R group (P<0.01); While MDA levels were lowerin sinomenine groups than that in I/R group (P<0.01).And abnormal morphological changes of liverduring I/R procedure were remarkable in sinomenine groups.Conclusions:Sinomenine was good for the hepatic injury of ischemia/reperfusion by inhibiting the production of oxygenic free radicals and improving the function of microcirculation.更多还原