【摘要】 次传代得到较纯的MSCs,倒置相差显微镜下观察细胞形态,应用免疫细胞化学方法对细胞的表面抗原标志进行检测。取3代MSCs,B-ME诱导6小时候后,倒置相差显微镜下观察细胞形态,应用免疫细胞化学方法鉴定诱导后细胞的表型特征。结果:倒置相差显微镜观察发现,接种后24h,细胞开始贴壁。培养3代以后,细胞呈梭形或多角形,折光性好,核为圆形、居中。免疫细胞化学检测细胞表面抗原显示:CD34-、CD45-、CD29+、CD44+、CD90+。诱导后细胞发出突起,逐渐生长延伸并彼此相连,形态学上具有神经细胞的明显特征。免疫细胞化学显示诱导后细胞NSE(神经元特异性烯醇化酶)阳性。结论:所采用的细胞分离培养方法简便可行,所获得的细胞其表型特征与文献报道一致,且具有向神经细胞分化的潜能.更多还原

【Abstract】 Objective:To culture rat MSCs and explore its biologic characteristics.Methods:The nucleated cells in rat long bone marrow tissue were separated by adhering.The MSCs were purified by discarding suspended cells through passage.The morphology of cultured MSCs was observed under inverted phase contrast microscope.For identifing MSCs,cell surface antigens were detected by immunocytochchemistry staining.Take the MSCs of passage 3,after induced by B-ME for 6h,the morphology of those cells were observed under inverted phase contrast microscope and the phenotypic characteristics of those cells were observed by immunocytochchemistry staining.Results:Under microscope,the cells started ahead of 24h.After three passage,the attached cells were displayed spindle or polygons and reflected light well,the nuclea is round shape and center.Immuophenotye studies presented that the cultured MSCs were CD29,CD44,CD90 positive and CD34,CD45 negative.After induced by B-ME,the processes of Cells spreaded and connected with each other,displaying morphologic features of neurocyte.Immunocytochchemistry staining showed that the cells after induced NSE-positive.Conclusions:The results suggest that it is easy and feasible to dissociate and culture rat MSCs.The MSCs can be identified by immuophenotye study and have the potential of neural differention.更多还原