【摘要】 对Rhizopus microsporus var.chinensis固态发酵产生淀粉酶的条件进行了优化。首先采用单因子实验确定最适固态发酵基质为小麦麸皮,最适碳源和氮源分别为可溶性淀粉和硫酸铵,通过控制培养基初始pH为3.0、初始湿度为70%时,使其生淀粉酶产量提高3.5倍。在此基础上,利用响应面中心组合设计对显著因素进行优化,得出每500mL三角摇瓶中含小麦麸皮13.7g、可溶性淀粉0.063g、硫酸铵0.052g时,生淀粉酶产量达到48.50U/mL,比初始产量提高了8倍。更多还原

【Abstract】 The fermentation condition of raw starch digesting amylase by Rhizopus microsporus var. chinensis was optimized. Using single factorial experiments,sources of solid state substrate,carbon and nitrogen were determined as wheat,soluble starch and ammonium sulfate,respectively. Then raw starch digesting amylase production was increased by 3. 5 times in the above medium with initial pH of 3. 0 and humidity of 70%. The optimal medium composition for raw starch digesting amylase production was determined by response surface methodology as:13. 7g wheat,0. 063g soluble starch and 0. 052g ammonium sulfate in 500mL flask. The enzyme production was increased to 48. 50U/mL,which was 7 times higher than that of the original medium.更多还原