【摘要】 对柠檬酸生产菌种黑曲霉进行了85℃高温和亚硝基胍复合诱变研究,确定了黑曲霉单孢子悬液适宜的诱变条件,温度为85℃高温,水浴时间为10min,质量浓度为1mg/mL亚硝基胍溶液的处理时间为4min,通过对复合诱变后的菌株进行平板初筛和摇瓶复筛,获得了一株遗传性能稳定的优良菌株TN-02,平均产酸率为13.61%,与出发菌株相比,产酸增幅达到43.20%。更多还原

【Abstract】 For the breeding of Aspergillus niger for nitric acid fermentation,the relationship between the survival rate and mutagenic time were researched,the results showed the optimal time for 85℃,mutation bath time was 10 min, and 4min for 1mg /mL NTG mutation.In addition,by using 85℃ and NTG compound mutation,high-yielding and genetic stability strains TN-02 was obtained,acidogenic rate reached 13.61% and raises 43.2% compared to that of the original strain.更多还原