【摘要】 目的研究羟基红花黄色素A(HSYA)对离体大鼠左心室收缩功能的影响。方法 42只大鼠根据实验需要随机分为正常对照组、HSYA组(1～100μmol/L)、HSYA(30μmol/L)对照组和HSYA(30μmol/L)+K+通道阻断剂组(分别为KATP阻断剂Gli、BKCa阻断剂Tea、KV阻断剂4-AP和KAch阻断剂Art),每组6只。经Langendorff离体心脏恒压灌流,依次梯度给予含有HSYA的灌流液。通过左心室内的乳胶水囊经换能器连于PowerLab生物信号采集分析系统,观察给药前后以及不同浓度HSYA对左心室收缩功能的影响,并观察不同的K+通道阻断剂对HSYA作用的影响。结果 HSYA能浓度依赖性抑制左心室收缩压、舒张末压、收缩最大速率和心率(P<0.05或P<0.01);对舒张最大速率影响不大(P>0.05)。Gli及Tea能剂量依赖性阻断HSYA的效应(P<0.05或P<0.01),4-AP及Art对HSYA的效应没有影响(P>0.05)。结论 HSYA可呈剂量依赖性抑制心脏的收缩功能和心率,其作用机制与开放KATP和BKCa通道有关。更多还原

【Abstract】 Objective To investigate the effects of hydroxy safflor yellow A(HSYA)on contractile function of left ventricle of isolated rat heart.Methods Forty-two rats were randomly divided into control group,HSYA(1-100 μmol/L)group,HSYA(30 μmol/L)control group,HSYA(30 μmol/L)+glibenclamide(Gli)group,HSYA(30 μmol/L)+tetraethylammonium(Tea)group,HSYA(30 μmol/L)+4-aminopyridine(4-AP)group and HSYA(30 μmol/L)+atropine(Art)group,with 6 rats in each group.Langendorff perfused heart preparation was made,and the isolated hearts were perfused with HSYA in a stepwise fashion.The effects on contractile function of left ventricle before and after treatment with different concentrations of HSYA were observed by PowerLab system from a fluid filled latex balloon in the left ventricle.The influence of different types of K+ channel blockers on the effects of HSYA was also observed.ResultsHSYA reduced left ventricular systolic pressure,left ventricular end-diastolic pressure,maximum rate of increase of left ventricular pressure and heart rate in a dose-dependent manner(P<0.05 or P<0.01),while had no significant effect on maximum rate of decrease of left ventricular pressure(P>0.05).Gli and Tea blocked the effects of HSYA in a concentration-dependent manner(P<0.05 or P<0.01),but 4-AP and Art did not block the effects of HSYA(P>0.05).Conclusion HSYA could significantly decrease the myocardial contractility and heart rate in a dose-dependent manner,and its action mechanism might be related to the opening of ATP sensitivity K+ channel and Ca2+ dependent K+ channel.更多还原