【摘要】 目的研究DEK和Ki-67基因蛋白检测对宫颈病变的临床病理学意义。方法20例正常宫颈上皮、83例宫颈上皮内瘤变(cervical intraepithelial neoplasia,CIN)、87例宫颈鳞状上皮癌(squamous cell carcinoma,SCC)和32例宫颈腺癌(adenocarci-noma)共222例存档蜡块选自延边大学医院、延边妇幼保健院和延边肿瘤医院病理科。应用组织芯片和免疫组化方法检测DEK和Ki-67蛋白在上述病变组织中的表达。结果20例非肿瘤性的正常宫颈上皮组织标本中DEK和Ki-67蛋白表达阴性,而DEK蛋白和Ki-67蛋白的阳性表达率在SCC中分别为82.8%(72/87)和88.5%(77/87),在子宫颈腺癌标本中分别为84.4%(27/32)和93.8%(30/32),而在CIN-1标本中分别为54.3%(19/35)和57.1%(20/35),在CIN-2标本中分别为69.2%(18/26)和76.9%(20/26),在CIN-3标本中分别为77.3%(17/22)和81.8%(18/22)。DEK和Ki-67蛋白均与宫颈癌分级和分期不相关。结论DEK蛋白检测可以作为宫颈癌细胞的增殖指标之一,与Ki-67蛋白有着相似的阳性表达部位和表达率,可作为宫颈癌的早期诊断指标,并有望成为宫颈癌靶向治疗的新靶点。更多还原

【Abstract】 Purpose To investigate the clinicopathological significance of DEK and Ki-67 protein over-expression in uterine cervical lesions.Methods 20 cases of non-neoplastic cervix with or without squamous metaplasia,83 cervical intraepithelial neoplasia,87 squamous cell carcinomas(SCCs),and 32 adenocarcinomas were selected for immunostaining of DEK and Ki-67.And the tissue microarray was also made for the immunohistochemical staining.Results DEK and Ki-67 were negative in all 20 cases of non-neoplastic cervical epithelia,however,the positive rates of DEK and Ki-67 were 82.8%(72/87) and 88.5%(77/87) in SCCs,84.4%(27/32) and 93.8%(30/32) in adenocarcinomas,54.3%(19/35) and 57.1%(20/35) in CIN-1,69.2%(18/26) and 76.9%(20/26) in CIN-2,and 77.3%(17/22) and 81.8%(18/22) in CIN-3,respectively.Meanwhile the expression of both DEK and Ki-67 proteins was not correlated with the grade and clinical stage of cervical cancer.Conclusions DEK could be a marker of cell proliferation as does Ki-67 in positive rate and staining pattern in uterine cervical cancers,suggesting that DEK might be a biomarker for early diagnosis and a potential therapeutic target of cervical cancers.更多还原