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光色素荧光探针测定抗体蛋白质

Determination of Antibody Protein with Lumichrome Fluorescence Probe

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【Author】 WANG Li-li2,ZHUANG Hui-sheng1,2,ZHOU Chun2(1.School of Environmental Science and Engineering,Shanghai Jiaotong University,Shanghai 200240,China;2.College of Environmental Science and Engineering,Dong-hua University,Shanghai 201620,China;3.Taizhou Municipal Central Station of Environmental Monitoring,Taizhou 318000,China)

【机构】东华大学环境科学与工程学院；上海交通大学环境科学与工程学院；台州市环境监测中心站；

【摘要】由于牛血清白蛋白(BSA)与光色素的结合反应,引起光色素荧光淬灭,且其荧光淬灭程度与BSA的浓度在0.050～1.0 mg.L-1范围内呈线性关系。据此提出了以光色素为探针测定抗体蛋白质的荧光方法。方法的检出限(3S/N)为8.0μg.L-1。用标准加入法对方法的回收率作了试验,测得回收率在95.0%～104.0%之间,测定值的相对标准偏差(n=6)小于2.5%。更多还原

【Abstract】 Based on the fluorescence quenching of lumichrome due to its combination with bovine serum albumin(BSA) and the linear relationship between magnitude of intensity of fluorescence quenching of lumichrome and concentration of BSA in the range of 0.050 to 1.0 mg·L-1,a fluorospectrophotometric method for determination of BSA using lumichrome as fluorescence probe was proposed.Detection limit(3S/N) of the method was found to be 8.0 μg·L-1.Recovery and precision of the method were tested by the standard addition method,giving values of recovery in the range of 95.0%-104.0%,and RSD′s(n=6) less than 2.5%.更多还原

【关键词】荧光光谱法；荧光探针；光色素；抗体蛋白质；牛血清白蛋白；
【Key words】 Fluorospectrophotometry； Fluorescence probe； Lumichrome； Antibody protein； BSA；

【基金】上海市基础研究基金重点项目(O9JC1407600);上海市科技攻关基金项目(09231202805);上海市重点学科建设项目资助(编号:B604)

【文献出处】理化检验(化学分册) ,Physical Testing and Chemical Analysis(Part B:Chemical Analysis) , 编辑部邮箱 ,2010年11期
【分类号】Q51-3
【下载频次】172

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