【摘要】 过氧化氢酶能催化过氧化氢的分解,在食品、环保、纸浆和造纸业、以及纺织行业具有重要的工业应用价值。研究从堆肥中分离了一株产过氧化氢酶的嗜热菌。该菌最适生长温度为55℃,最适生长pH值为7.0,最适NaCl浓度为1.5%。通过16S rDNA编码基因序列分析,该菌和环状芽孢杆菌有较高的同源性,命名为Bacillus sp.SHT2。通过对培养基中的碳、氮源进行优化,酶活达到196.9 U/mL。酶学性质研究表明该菌产生的过氧化氢酶具有良好的热稳定性、pH稳定性和储存稳定性。更多还原

【Abstract】 As one kind of oxidases,catalases can catalyse the decomposition of the cellular hydrogen-peroxide and thus is of important potential in the fields of food and dairy industry,environmental protection,paper pulp and paper making industry and textile industry.A thermophilic strain with high catalase activity designated SHT2 was isolated from the composting.The suitable cultivation temperature,pH and NaCl concentration for this bacterium is respectively 55℃,7.0 and 1.5%.Based on the analysis of the 16S rDNA sequence,this strain was identified as Bacillus sp SHT2.Through optimization of C,N source in substrate,the catalase activity of the Bacillus sp.SHT2 reached 196.9 U/mL.The optimum conditions of this catalase are at temperature 50℃and pH 7.0 respectively. The catalase is stable below 60℃and at pH range of 4—7.更多还原