【摘要】 为探索卵巢局部瘦素信号缺失对卵巢自身功能的影响,进行以下研究:①对瘦素长受体(LR)缺失(LR-)的雌性B6.Cg-m+/+Leprdb小鼠及其同窝出生的有完整LR的雌性野生型C57BL/6J小鼠(LR+)进行卵巢交互移植,在同一雌性野生型C57BL/6J小鼠体内构建两侧卵巢不同的LR基因型,分别标记为KO(卵巢LR-)、WT(卵巢LR+)两组。②监测卵巢的周期变化;动情周期恢复后各组均予GnRH-a降调节后取材,RT-PCR检测分子生物学指标。结果显示:①卵巢移植术后小鼠仍有正常的动情周期,经GnRH-a降调节后,小鼠失去动情周期的变化;②RT-PCR结果显示,KO组卵巢中Star、Cyp17、Cyp19、Jak2、Stat3、Pias3mR-NA的表达量均明显低于WT组,Ob-RbmRNA在KO组卵巢中无表达,WT组有表达,Socs3mRNA在两组间表达无明显差异。研究表明,卵巢内存在完整的瘦素信号传导通路;瘦素受体缺失及外周糖脂代谢环境对卵巢功能的影响并不重要,重要的是下丘脑-垂体对卵巢的调控作用,其对卵巢作用的分子机制是调控卵巢甾体激素合成酶基因的表达。更多还原

【Abstract】 To study the effects of impaired leptin signaling function on the ovary reproductive failure, which is supposed to be related with the interaction of leptin and reproduction, adult female C57BL/6J mice were used. Littermate wide types (LR+), as well as diabetic (db/db) mutant genotypes (LR-), were matched for the allograft of the ovary. Female C57BL/6J mice with different genotype ovaries were planted with ovary LR - as Group KO, with ovary LR + as Group WT. The vaginal smear was used to evaluate the recovery and cyclicity of transplanted ovaries. During the cycle recovery, each mouse was administered with GnRH-a to down-regulation, and then the mice without cyclicily were executed. The ovaries were collected for RT-PCR for the assay. It is shown that all mice recover estrous cycle. Ovaries’ structure is recovered in Group KO, and without weight difference as compared to Group WT. RT-PCR shows that Ob-Rb is negative in ovary of group KO ,and is positive in ovary of group WT. The difference of gray scale in ovary of group KO shows that Star, Cyp17, Cyp19, Jak2, Stat3, Pias3 mRNA are lower than those in group WT. However, Socs3 mRNA shows no significant difference in two groups. The above results indicate that Leptin signal transduction system exists in the ovary. And defective leptin receptor within ovaries and the milieu of glucolipid metabolism are not important to its own function. Hypothalamus and pituitarium regulation is critical to ovaries reproductive function. The molecular mechanism is to regulate the gene expression of steroid hormone synzyme.更多还原