【摘要】 探讨不同浓度H2O2对人肝癌细胞株Hep-G2细胞凋亡的影响.使用不同浓度(0.01、0.05、0.10、0.20、0.50mmol/L)的H2O2作用于Hep-G2细胞12h,通过丫啶橙-碘化丙啶(AO/PI)双重荧光染色法对Hep-G2细胞的形态变化进行观察;再进行DNA的提取和琼脂糖凝胶电泳观察DNA断裂情况.结果表明:不同浓度的H2O2处理后在荧光显微镜下可以区分正常对照细胞(0mmol/L)、染色质凝聚的早期凋亡细胞(0.01、0.05mmol/L)、细胞质皱缩和细胞核解体的晚期凋亡细胞(0.10、0.20mmol/L)和降解细胞(0.50mmol/L),在一定时间内随H2O2浓度的增加而增大,呈现浓度依赖性.DNA电泳结果显示发生凋亡的细胞基因组DNA形成弥散条带,从而表现出体外细胞凋亡的特征.因此,H2O2可以诱导Hep-G2细胞凋亡.更多还原

【Abstract】 To investigate the effects of different concentrations of hydrogen peroxide on apoptosis in Hep-G2 cells,Hep-G2 cells were treated 12 hours by different concentrations of hydrogen peroxide that were 0.01、0.05、0.10、0.20、0.50mmol/L and the acridine AO/PI double staining fluorescence was used for Hep-G2 cells line to detect the morphological changes of apoptotic cells under fluorescence microscope.Genomic DNA of Hep-G2 cells was extracted for testing base pair fragments.The results showed the cells could be classificated into: normol cells(0mmol/L);early apoptotic cells with chromatin agglomerating(0.01,0.05mmol/L);last apoptotic cells with cytoplasm buckling and cell nucleus disassembly(0.10,0.20mmol/L) and degradationed cells under the fluorescence microuscopy,and in a certain period they increased with increasing concentration of H2O2 which showed dose-dependent.The genomic DNA of apoptotic Hep-G2 cells could be degradated into disperse DNA bands,which showed the characteristics of cell apoptosis in vitro.Therefore,human hepatoma cells Hep-G2 cell could be induced to apoptosis by H2O2.更多还原