【摘要】 试验在魔芋DNA不同提取方法比较的基础上,利用RAPD技术对谢君魔芋不同变异植株的DNA进行了检测。结果表明,利用改良的CTAB法可以得到纯度高的DNA样品,利用8条随机引物可以鉴定出谢君魔芋的变异。对9株谢君魔芋复叶分裂方式、气生球茎生成个数、叶柄色泽(斑纹)不同变异系的检测结果表明,其RAPD扩增结果均有差异,变异系间的相似性系数分布范围在0.440～0.800,平均相似性系数为0.714,其中植株1与植株8的相似系数最大,为0.800,植株1与植株3的相似系数最小,为0.440。采用UPGMA法进行聚类分析,9株谢君魔芋变异株归于3类,并建议在谢君魔芋变异株系选择上可将叶柄色泽(斑纹)作为主要选择指标。更多还原

【Abstract】 In this research, a reliable DNA extraction from different variety of Amorphophallus xiei was developed for RAPD analysis.The results revealed that the high quality DNA was extracted by the modified CTAB method and 8 random primers were used to identify the variety.The way of leaf dividing, the number of bulb and the strips of leafstalk in 9 varieties were analyzed.The results showed the Nei’s similarity of coefficients were in the range of 0.440～0.800, the average Nei’s similarity of coefficient was 0.714, the maximal similarity of coefficient was 0.008 between clone 1 and clone 8, the minimal Nei’s similarity of coefficent was 0.440 between clone 1 and clone 3.These 9 varieties were clustered into 3 groups by UP-MGA analysis.The strips of leafstalk were suggested to be the main index for selected variety in Amorphophallus xiei.更多还原