【摘要】 在林间喷施不同浓度的松毛虫质型多角体病毒(CPV)2.8×107PIB/ml、2.8×106PIB/ml、2.8×105PIB/ml15天后,调查结果表明:三种浓度的CPV感染马尾松毛虫后中肠发白率分别达到72.2%、69.2%、20.2%。林间给马尾松毛虫自然种群喷施2.8×106PIB/mlCPV增殖病毒,经济有效。开展了大规模CPV提取试验,提取的CPV制剂多角体达25.2×108PIB/g,病毒提取率6.8%。进行了CPV保存试验,试验表明低温有利于CPV保存。更多还原

【Abstract】 Different concentrations(2.8×107PIB/ml、2.8×106PIB/ml、2.8×105PIB/ml) of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus(DpCPV) were sprayed in the forest,and after 15 days,infection rates of Dendrolimus punctatus larvae were 72.2﹪、69.2﹪、20.2﹪,respectively.This result showed that spraying of DpCPV with concentration of 2.8×106PIB/ml is cost-effective.Furthermore,extraction experiment of DpCPV was conducted and,as a result,25.2×108PIB/g of DpCPV was obtained,with an extraction rate of 6.8﹪.This study also showed that lower temperature is suitable for the preservation of DpCPV.更多还原