【摘要】 以2a生山楂(Crataegus pinnatifida Bge.)为试材,研究水培条件下缺素处理对山楂根系分泌液和山楂根系中黄酮总量及单体黄酮种类的影响。结果表明,缺氮、缺磷处理能够提高根系分泌液中的总黄酮含量,但是差异不显著。缺锌处理的山楂根系中的黄酮种类最多,为5种已知黄酮成分,即牡荆素鼠李糖苷、芦丁、牡荆素、金丝桃苷、槲皮素。对照、缺磷、缺钾、缺钙、缺镁、缺铁中只检测到3种已知黄酮成分,即芦丁、金丝桃苷、槲皮素。缺氮检测到2种已知黄酮成分,即金丝桃苷和槲皮素。在缺镁、缺锌与缺铁处理的山楂根系分泌液中,均检测到5种已知黄酮成分。而对照、缺氮、缺磷、缺钾、缺钙处理只检测到少数几种已知黄酮成分,且含量相对偏低。在山楂的黄酮调控方面,适当的进行缺素处理能够增加黄酮的总量或种类。更多还原

【Abstract】 The effects of mineral deficiency on total flavonoid content and monomer flavonoid of hawthorn roots and root exudates were studied using two-year old hawthorns cultured in nutrient solution as materials. The results indicated that the total flavonoid contents of root exudates in No-N and No-P treatments were enhanced. The monomer flavonoids of roots were the most in No-Zn treatment, and five known flavonoids including rhamnosylvitexin, rutin, vitexin, hyperoside and quercetin were detected, while three known flavonoids including rutin, hyperoside and quercetin were detected under No-P, No-K, No-Ca, No-Mg, No-Fe treatment and control, however, only two flavonoids that were hyperoside and quercetin were detected in No-N treatment. For root exudates of hawthorn, five known flavonoids that were rhamnosylvitexin, rutin, vitexin, hyperoside and quercetin were detected under No-Mg,No-Zn and No-Fe treatments, while only a few flavonods were detected in No-N, No-P, No-K, No-Ca treatments and control, and the content was lower. For the flavonoid regulation, proper mineral deficiency treatment can increase the contents of total flavonoid and monomer flavonoids.更多还原