【摘要】 Pi-1是具有广谱抗性的水稻显性主效抗稻瘟病基因,位于水稻11号染色体末端。本研究选择靠近此处的SSR标记15对,用含有Pi-1基因的Lac23与不含Pi-1基因恢复力强的N优69-1进行检测。检测出有差异条带5对引物,分别为RM224、RM254、RM2136、RM6094和RM6293。另外,以N优69-1为母本,以LAC23为父本进行杂交至F8代群体,利用上述差异分子标记,在F8趋亲单株中选择与Lac23标记一致的单株,与田间稻瘟病鉴定结果相比较。结果显示,RM6293对含有Pi-1基因材料的选育有较大的准确度,其次为RM254>RM224>RM2136>RM6094。本实验与其它研究不同的是采用了F8代群体,认为多次自交重组后的遗传稳定株系才可能得到稳定的标记。更多还原

【Abstract】 The rice blast,caused by fungus Magnaporthe grise,is one of the most devastating rice diseases,and Pi-1 gene is one of the major effective gene in rice which have the possession of broad-spectrum resistance to rise blast.It had been mapped on the bottom of rise chromosome 11.In this research,15 pairs of SSR marker which tightly linked to Pi-1 gene were selected to detect if there are strip difference between Lac23 and Nyou69-1,while the Pi-1 gene distributed in Lac23 and Nyou69-1 get the strong resilience with lower resistance.The result showed that there are five marker polymorphism(RM224,RM254,RM2136,RM6094,RM6293) between them.An F8 population including 297 individuals developed from a cross between Lac23 and Nyou69-1 has been identified with SSR marker.Compared with field trial,the result showed RM6293 has higher accuracy to breeding the individuals with rice blast resistance gene Pi-1.Then the following orderare RM254>RM224>RM2136>RM6094.In this research,we chose F8 population as materials,and considered that stable markers could only be obtained from genetic stable lines.更多还原