【摘要】 目的探讨利用房水中钾离子浓度进行死亡时间推断的可行性。方法30只家兔随机分为10组,以空气栓塞法处死后,于不同的死亡时间点分别采集房水和玻璃体;用Vitros 950干式自动生化仪对所有样本中的K+、Na+、Ca2+三种离子进行浓度测定;用SPSS统计软件对测定数据进行回归分析。结果房水中钾离子浓度变化与死亡时间呈高度相关,对应的回归方程为:PMI=1.572-0.597×[K+]+0.107×[K+]2-0.002×[K+]3。其相关系数(R2=0.95)明显高于同批样本的玻璃体钾离子浓度与死亡时间的相关系数(R2=0.5)。房水、玻璃体液的Na+、Ca2+浓度与死亡时间的相关性不明显。结论利用房水钾离子浓度推断死亡时间可获得较好的准确度。更多还原

【Abstract】 Objective To investigate the applicability of the K+ concentration in aqueous humor to estimate postmortem interval(PMI).Methods 30 White New-Zealand rabbit were sacrificed by air embolism and divided into 10 groups.Aqueous humor and vitreous humor were sampled at 0.5h,1h,3h,6h,8h,12h,16h,20h,and 24h after death.The concentration of potassium,sodium and calcium were analyzed by an auto-analyzer and the data were statistically processed by SPSS software for Windows.Results Increase in potassium concentration in aqueous humor was correlated with the postmortem interval(R2=0.956).Conclusion Measurement of potassium concentration in aqueous humor may be used for PMI estimation.更多还原