【摘要】 将秦皇岛某养鸡场疑似为传染性法氏囊病的病死鸡进行剖检,为了对该疑似病例进行确诊,并进行流行病学研究,经对流免疫电泳,对采集的病料进行病毒的分离,阳性法氏囊病料进行病毒RNA的提取,采用套式RT-PCR对病毒的VP2高变区进行扩增,分析氨基酸序列。结果表明,该分离株为传染性法氏囊病病毒(IBDV),被检毒株与GenBank报道的标准超强毒株OKYM、UK661、DV86同源性高达100%,与V3、V2亲缘关系较近。动物回归试验可使健康鸡100%发病,病死率80%。表该分离株为鸡传染性法氏囊病病毒超强毒株。更多还原

【Abstract】 Diagnosis and epidemiologic study were carried on the dead chicken with suspected infectious bursal disease in a Qinhuangdao chicken farm.The virus was isolated from the chicken with counter immunoelectrophoresis and the virus RNA was extracted.IBDV VP2 variable region was amplified by nested RT-PCR and the amino acid sequence was analyzed.Results showed that the isolated strain was IBDV.Sequence comparison indicated that the strain belonged to very virulent IBDV,which sharing 100% homology wth OKYM,UK661 and DV86 and had similar genetic relationship with V3 and V2.Animal regression experiment showed that all the health chickens were sick and 80% died after infection with the virus.更多还原