【摘要】 目的茶树菇蛋白具有体外降压活性,以水浸提法提取茶树菇降压活性蛋白、优化提取工艺。方法采用单因素实验考察了液固比、提取时间、提取温度和提取液的pH等因素对茶树菇降压活性蛋白提取率及提取产物对血管紧张素转化酶(ACE)体外抑制效果的影响。结果降压活性蛋白提取率及浸提产物对ACE抑制效果最佳条件是:液固比为40∶1,提取时间3 h,提取温度为30℃,提取液初始pH为8.0,此时活性蛋白质得率最高可达到87.7%,提取物对ACE的抑制活性可达54.0%。结论本提取工艺是一种操作简单、效率较高的茶树菇降压活性蛋白提取工艺。更多还原

【Abstract】 Objective To optimize the techniques for extracting hypotensive active peptides from Agrocybe aegerita.Method The effects of the liquid/solid ratio,extraction time and temperature,pH value of the initial liquid on the extraction percentage(EP) of the hypotensive active peptides were investigated,and inhibition percentage(IP) of the extracts on angiotensin I-converting enzyme was determined.Results Optimal extraction of the hypotensive active peptides from Agrocybe aegerita was achieved with the liquid/solid ratio of 40:1,extraction time of 3 h,extraction temperature at 30 ℃,and pH=8 of the initial liquid.The EP of the hypotensive active peptides from Agrocybe aegerita could reach 87.7% with IP of the extracts on angiotensin I-converting enzyme of 54.0%.Conclusion This method is simple and efficient for extracting hypotensive active peptides from Agrocybe aegerita.更多还原