【摘要】 为更好地将RAPD技术应用于梅花品种资源的鉴定以及遗传基础的研究,以16个梅花品种为实验材料,对琼脂糖凝胶电泳以及聚丙烯酰胺凝胶电泳(PAGE)检测RAPD的PCR扩增产物的效果进行了比较,结果表明PAGE检测出谱带的总数量以及多态性谱带的数量均高于前者。根据2种电泳系统获得的标记信息构建的树状聚类图表明,PAGE检测的结果与传统梅花的分类情况完全一致。通过对随机挑选的25个片段进行克隆与测序的结果发现,25个片段都是对应引物的RAPD扩增产物。其中有4条是编码蛋白的基因片段,说明RAPD不仅扩增基因组上的非编码蛋白序列,而且可以扩增编码蛋白的基因片段。更多还原

【Abstract】 In order to utilize RAPD better in the identification of mei(Prunus mume) cultivars and mei genetic analysis,agarose and polyacrylamide gel electrophoresis(PAEG) were used to verify RAPD amplified products of the genomic DNA of 16 mei cultivars.Their results were compared.The results showed that the polyacrylamide gel electrophoresis for RAPD amplified fragments of P.mume presents more bands and more polymorphic bands than agarose gel electrophoresis.The dendrograms derived from the analysis of RAPD results were consistant to the traditional classification based on morphological traits.The random sequencing of 25 fragments showed that all the fragments were of PCR products of RAPD from the corresponding primer,among which four sequences were regions of encoding protein genes,suggesting RAPD can amplify both protein-encoding and non-protein encoding gene sequences.更多还原