【摘要】 [目的]建立用重组蛋白Ⅸ检测犬传染性肝炎病毒抗体的间接ELISA方法。[方法]分别设置不同抗原包被浓度、包被条件、封闭液等,比较ELISA反应效果。[结果]最佳抗原包被浓度为5.0μg/ml;最佳包被条件为,37℃包被1 h、4℃过夜;最佳封闭液为5%BSA;HRP-羊抗犬IgG的最佳工作浓度为1∶1 600。[结论]建立了检测犬传染性肝炎病毒抗体的间接ELISA方法。更多还原

【Abstract】 [Objective]To establish an indirect ELISA for detecting of CAV-Ⅰ antibody by Protein Ⅸ.[Method]Different coating concentrations of antigen,different coating conditions,different closed reagents,et al were designed,and the results of ELISA were compared.[Result]The optimal coating concentration of antigen was 5.0 μg/ml.The optimal coating condition was 4 ℃ overnight after coating at 37 ℃ for 1 h.The optimal closed reagent was 5% BSA.The optimal working condition of HRP-coating antigen IgG was 1∶1 600.[Conclusion] An indirect ELISA for detecting CAV-I antibody by Protein Ⅸ was established.更多还原