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匀浆法提取——高效液相色谱法测定烟草料液中苯甲酸·山梨酸
Study on the Determination of Benzoic acid and Sorbic acid in Tobacco Sauce By Homogenate Extraction-High Performance Liquid Chromatography
【摘要】 [目的]研究了匀浆法提取-高效液相色谱法测定烟草料液中的苯甲酸和山梨酸。[方法]用匀浆法提取料浸样品,然后采用WatersOasis(HLB固相萃取小柱预分离。以Waters-XbridgeShieldRP18(3.9×150mm,5μm)色谱柱为固定相,0.2%磷酸和乙睛为流动相,流速1.2ml/min。苯甲酸和山梨酸在10min内可达到基线分离,并采用紫外二极管矩阵检测器检测。[结果]方法标准回收率为92%~104%,相对标准偏差为2.8%~3.2%,结果满意。[结论]该研究方法重现性好,回收率较高,检出限较低,可用于烟草料液中苯甲酸和山梨酸含量分析的推广。
【Abstract】 [Objective]The aim was to study determination of benzoic acid and sorbic acid in tobacco sauce by homogenate extraction-high performance liquid chromatographg.[Method]The tobacco sauce samples were extracted by high-speed homogenization with water,and then purified by solid phase extraction with Waters OasisHLB cartridge.The benzoic acid and sorbic acid were separated on a Waters-Xbridge Shield RP(3.9×150 mm,5 μm) chromatographic column,with acetonitrile and 0.2% phosphoric acid (20/80) as mobile phase at a flow-rate of 1.2 ml/min,and detected with the photodiode array detector at 250 nm.The benzoic acid and sorbic acid could get a baseline separation within 10 min.[Results] The recoveries of the benzoic acid and sorbic acid were 92%-104%,and relative standard deviations were 2.8%-3.2 %.[Conclusion] This method was suitable to widely apply to the determination of benzoic acid and sorbic acid in tobacco sauce with good reproducibility,high recovery and low detection limit.
【Key words】 High performance liquid chromatography; Solid phase extraction; Benzoic acid; Sorbic acid; Tobacco sauce;
- 【文献出处】 安徽农业科学 ,Journal of Anhui Agricultural Sciences , 编辑部邮箱 ,2010年11期
- 【分类号】S572
- 【被引频次】6
- 【下载频次】167