【摘要】 目的研究体外过氧化氢对生育男性精子凋亡及精子DNA完整性的影响。方法58例正常生育男性均来自吉林大学第二临床医院泌尿男科,精子Percoll优选后制作精子悬液,用伊红Y染色分析精子活率,用瑞-姬染色分析精子凋亡率,用精子核吖啶橙荧光染色检测精子DNA完整性。结果外加过氧化氢浓度为0·02mmol/l时,精子活率显著低于对照组(P(0·05)。当浓度为0·20mmol/l时,精子活率显著低于对照组(P(0·05),精子凋亡率显著高于对照组(P(0·05)。外加过氧化氢浓度为0·02mmol/l、0·2mmol/l时,精子DNA完整性均显著低于对照组(P(0·05),且精子DNA完整性随实验浓度增加而下降。结论体外过氧化氢对精子活率、凋亡率与DNA完整性有影响,高浓度的过氧化氢可促使精子凋亡,损伤精子DNA完整性,导致男性不育。更多还原

【Abstract】 Objective:To study effect of H2O2 in vitro on human sperm viability and DNA integrity of fertile male. Methods:Semen samples were obtained from 58 fertile male in department of urology,the second hospital,Jilin University. Sperm viability was detected by eosin-Y staining. The semen smears were stained by Wright-Giemsa staining for analyzing spermapoptosis rate. Sperm DNA integrity was detected by sperm nucleus acridine orange fluorescence staining. Results:When H2O2 concentration in vitro was 0.02mmol/l,sperm viability was markedly lower than that of control group(P﹤0.05). When it was 0.02mmol/l,sperm viability was markedly lower than that of control group(P﹤0.05),sperm apoptosis rate were markedly higher than that of control group(P﹤0.05). When it were 0.02mmol/l,0.2mmol/l,sperm DNA integrity was both markedly lower than that of control group(P﹤0.05). With H2O2 concentration increasing,sperm DNA integrity was descending. Conclusion:H2O2 in vitro influence on sperm viability,sperm apoptosis rate and DNA integrity. H2O2 of high concentration could make sperm apoptosis,and damage sperm DNA integrity.更多还原