【摘要】 目的探讨荧光原位杂交(FISH)与免疫组织化学(IHC)检测HER2基因状态的结果的一致性及HER2表达与其淋巴结转移的关系及意义。方法采用IHC技术检测68例乳腺浸润性导管癌HER2基因的表达,同时进行FISH检测。结果HER2表达(+)的28例标本中,其中27例经FISH检测无扩增,1例有扩增;HER2表达(++)的12例标本中,7例经FISH检测无扩增,2例有扩增,另3例为可疑;HER2表达(+++)的28例标本中,经FISH检测均扩增;FISH检测有扩增的30例标本中,26例发生了淋巴结转移;可疑的3例中有2例有淋巴结转移;无扩增的34例标本中只有6例发生了淋巴结转移。结论当HER2表达为(+)或(+++)时,IHC和FISH检测的一致性较高,而当HER2表达为(++)时,必须进行FISH检测;HER2的扩增与淋巴结的转移关系密切,呈正相关(p<0.05)。更多还原

【Abstract】 Objective To explore the relationship between HER-2 gene expression and lymph node metastasis by Fluorescence in Situ Hybridization(FISH),and to compare these results with those obtained by immunohistochemistry(IHC).Methods IHC and FISH were employed to determine the expression of HER-2.Results Among 28 samples with HER-2 expression score(+) by IHC,27 were negative for HER-2 expression by FISH and only one was positive.Among 12 samples with score(++) by IHC,7 were negative and 2 were positive by FISH,remaining 3 uncertain.All of the 28 samples with HER-2 expression score(+++) by IHC were also positive by FISH.In addition,26 of the 30 cases with positive HER-2 expression by FISH were identified with lymphatic metastasis;2 of the 3 uncertain cases were identified with lymph node metastasis;however,only 6 of the 34 cases with negative HER-2 expression by FISH were identified with lymph node metastasis.Conclusion A high consistent results are showed by IHC and IHC when HER-2 expression score is(+) or(+++).However,when the HER-2 expression score is(++),the results of these two methods are different,so FISH must be employed.Additionally,there is a positive correlation between HER-2 expression and lymph node metastasis(p<0.05).更多还原