【摘要】 目的:研究银杏叶提取物(EGb761)对黄曲霉素B1(AFB1)致大鼠肝细胞癌(HCC)发生发展的干预效果及其抗氧化作用。方法:实验大鼠按体质量随机分成3组:A组(AFB1组)、B组(AFB1+EGb761组)和C组(对照组)。分别于实验第14、28、42、55周进行肝活检并于第64周全部处死,观察大鼠肝脏癌前病变(γ-GT灶)、组织病理学及肝组织GSH-Px及MDA的变化。结果:在癌前期,第42及第55周时,B组单个γ-GT阳性灶的面积及灶总面积均显著小于相应时段A组的各项指标(P=0.000);动态观察定期肝脏活检的组织显示B组大鼠诱癌各时期肝脏损害均较A组轻,增生性病变发生亦较迟,HCC诱发率(26.92%)明显低于A组(76%)(P=0.000),对照组无肿瘤发生;B组GSH-Px活力较A组升高,而MDA含量较A组降低(P<0.05)。结论:EGb761能明显抑制AFB1诱发大鼠肝的癌前病变及延迟癌前病变向癌发展,这可能与其提高机体的抗氧化能力有关。更多还原

【Abstract】 Objective: To investigate the effect of Ginkgo biloba extracts(EGb761) on aflatoxin B1(AFB1)-induced hepatocarcinogenesis and its antioxidant activity in Wistar rats.Methods: 71 Wistar rats were randomly divided into three groups: AFB1(group A);AFB1+EGb761(group B),Control(group C).Rats in gurop A and B were injected with AFB1 through abdomen and the doses were 100-200 μg/kg,one to three times a week.Liver biopsy were performed in all rats during 14thw,28thw,42thw and 55thw,and were executed at 64thw.Gammaglutamyl transpeptidase-positive hyperplastic cell foci(γ-GT foci) and histopathology of the liver tissue were observed.The levels of malondialdehyde(MDA),as well as the activity of Glutathione peroxidase(GSH-Px) was examined.Results: At 42thw and 55thw,the γ-GT focus area(mm2/focus)and general area of foci(mm2/cm2)of group B were significantly smaller than that in group A(P=0.000).The incidence of hepatocelluiar carcinoma(HCC) in group B(26.92%)was significantly lower than that in group A(76%)(P=0.000).Group C didn’t have HCC development.EGb 761 markedly increased GSH-Px activity,reduced MDA levels(P<0.05).Conclusion: EGB761 shows effective inhibition to hepatocarcinogenesis induced by AFB1 in rats,which may be related to its antioxidant acctivity.更多还原