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正交试验优化提取板蓝根药材中核苷类成分的研究

Studies on extraction process of nucleosides for radix isatidis

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【作者】 肖慧; 刘清飞; 王义明; 罗国安; 郑亚玉;

【Author】 XIAO Hui1,2,LIU Qing-fei1,WANG Yi-ming1,LUO Guo-an1,ZHENG Ya-yu2(1 Institute of Biomedicine,Tsinghua University,Beijing 100084,China;2 Guiyang University of TCM,Guiyang 550002,China)

【机构】 清华大学生命科学与医学研究院; 贵阳中医学院;

【摘要】 目的:优化板蓝根提取工艺条件。方法:以尿苷、鸟苷、腺苷的总含量为指标,采用正交试验对板蓝根水提工艺的加水量、回流次数、回流时间进行研究。结果:最佳提取条件为:用6倍量水回流提取3次,每次1h,上述3种核苷转移率为90.02%。结论:本工艺3种核苷总量的提取转移率较高,且工艺稳定性好,重现性好。

【Abstract】 Objective:To optimize the extraction process for radix isatidis. Methods:The process was studied by orthogonal design with the total content of guanosine,uridine,and adenosine as the detective markers. Results:The optimized extraction condition was obtained. The crude material was extracted 3 times with 6 times volume of water for 1 h. The extraction rate of the three nucleosides was 90.02% in the process above. Conclusion:The high extraction rate of nucleosides was obtained with high stability and reproducibility.

【关键词】 板蓝根; 核苷; 提取工艺; 正交试验;
【Key words】 radix isatidis; nucleosides; extraction; orthogonal design;
  • 【文献出处】 中国新药杂志 ,Chinese Journal of New Drugs , 编辑部邮箱 ,2009年04期
  • 【分类号】R284.2
  • 【被引频次】4
  • 【下载频次】570
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